LOCUS       sequence-141516-       12363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            mRFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB454
FEATURES             Location/Qualifiers
     source          1..12363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..3583
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2906..3583
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     3304..3323
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3498..3521
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      3605..3857
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3891..3907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3891..3907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3904..3926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3915..3931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3939..3945,3946..3963,3964..3969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3984..4005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4039..4291)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4681..5475)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4792..4811)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5402..5421
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5496..5679)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5532..5711)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5885..5909
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6430..7221
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7467..8055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7956..7975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8209..8226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8241..8381
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8307..8326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8467..8489
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8725..8919
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8985..10058)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10487..11116)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggc ctcctccgag gacgtcatca aggagttcat
     2941 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3001 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     3061 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
     3121 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3181 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3241 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3301 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
     3361 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     3421 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
     3481 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3541 acagtacgag cgcgccgagg gccgccactc caccggcgcc taagcttaga gctcgaattt
     3601 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     3661 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     3721 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3781 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3841 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg
     3901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc
     4021 gcctatttct gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     4081 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     4141 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     4201 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     4261 agaaacttta ttgccaaatg tttgaacgat cggggatcat ccgggtctgt ggcgggaact
     4321 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt
     4381 cgccgccgac gccgttgatg tggacgccgg gcccgatcat attgtcgctc aggatcgtgg
     4441 cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc accttcgacc gcctgttccg
     4501 cagagatccc gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     4561 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     4621 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     4681 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     4741 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     4801 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     4861 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     4921 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     4981 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     5041 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     5101 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     5161 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     5221 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     5281 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     5341 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     5401 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     5461 atcttgttca atcatgcgaa acgatccaga tccggtgcag attatttgga ttgagagtga
     5521 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     5581 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     5641 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
     5701 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     5761 ataacgtgac tcccttaatt ctccgctcat gatgaattca gaaataaatt cagcctaatt
     5821 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     5881 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     5941 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     6001 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     6061 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     6121 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     6181 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     6241 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     6301 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     6361 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     6421 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     6481 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     6541 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     6601 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     6661 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     6721 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     6781 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     6841 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     6901 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     6961 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     7021 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     7081 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     7141 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     7201 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     7261 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     7321 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     7381 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7441 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8101 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8161 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8221 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     8281 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8341 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     8401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8461 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     8521 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     8581 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     8641 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     8701 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     8761 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     8821 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     8881 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     8941 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     9001 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     9061 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     9121 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     9181 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     9241 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     9301 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     9361 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     9421 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     9481 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     9541 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     9601 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     9661 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     9721 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     9781 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     9841 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
     9901 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     9961 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    10021 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    10081 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
    10141 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    10201 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    10261 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    10321 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    10381 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    10441 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    10501 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    10561 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    10621 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    10681 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    10741 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    10801 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    10861 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    10921 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    10981 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    11041 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    11101 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    11161 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    11221 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    11281 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    11341 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    11401 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    11461 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    11521 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    11581 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    11641 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    11701 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    11761 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    11821 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    11881 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    11941 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    12001 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    12061 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    12121 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    12181 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    12241 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    12301 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    12361 cct
//
