LOCUS       sequence-141519-       12006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            CaMV35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB502
FEATURES             Location/Qualifiers
     source          1..12006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2927..3179
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3213..3229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3213..3229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3226..3248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3237..3253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3261..3267,3268..3285,3286..3291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3306..3327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3363..3615)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3766..4791)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4843..5022)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5528..5552
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6073..6864
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7110..7698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7599..7618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7852..7869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7884..8024
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7950..7969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8110..8132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8368..8562
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8628..9701)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10130..10759)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc gttcaaacat
     2941 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3001 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3061 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3121 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3181 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3301 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc
     3361 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     3421 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     3481 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     3541 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     3601 aatgtttgaa cgatcgggga aattcggggg atcgatcccg cgaactgtgg acgagaactg
     3661 tgccaccaag cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg
     3721 gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct
     3781 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     3841 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     3901 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     3961 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     4021 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     4081 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     4141 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     4201 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     4261 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     4321 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     4381 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     4441 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     4501 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaatgccc caatgtcaag
     4561 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     4621 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     4681 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     4741 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcttattg
     4801 ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg
     4861 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     4921 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     4981 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     5041 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     5101 tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     5161 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     5221 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     5281 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     5341 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     5401 tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta
     5461 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     5521 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     5581 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     5641 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     5701 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     5761 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     5821 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     5881 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     5941 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     6001 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     6061 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     6121 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     6181 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     6241 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     6301 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     6361 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     6421 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     6481 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     6541 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     6601 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     6661 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     6721 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     6781 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     6841 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     6901 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     6961 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     7021 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7081 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7861 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7921 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7981 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     8041 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8101 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8161 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     8221 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     8281 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     8341 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     8401 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     8461 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     8521 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     8581 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     8641 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     8701 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     8761 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     8821 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     8881 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     8941 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     9001 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     9061 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     9121 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     9181 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     9241 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     9301 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     9361 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     9421 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     9481 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     9541 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     9601 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     9661 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     9721 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     9781 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     9841 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     9901 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     9961 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
    10021 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    10081 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    10141 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    10201 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    10261 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    10321 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    10381 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    10441 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    10501 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    10561 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    10621 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    10681 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    10741 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    10801 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    10861 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    10921 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    10981 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    11041 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    11101 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    11161 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    11221 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    11281 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    11341 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    11401 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    11461 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    11521 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    11581 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    11641 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    11701 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    11761 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    11821 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    11881 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    11941 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    12001 aaacct
//
