LOCUS       sequence-141522-       12735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB505
FEATURES             Location/Qualifiers
     source          1..12735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2912..2914,2915..2917,2918..3631)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2957..2978)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3218..3237)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3565..3586
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3656..3908
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3942..3958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3942..3958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3955..3977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3966..3982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3990..3996,3997..4014,4015..4020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4035..4056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4092..4344)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4495..5520)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5572..5751)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6257..6281
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6802..7593
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7839..8427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8328..8347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8581..8598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8613..8753
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8679..8698)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8839..8861
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9097..9291
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9357..10430)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10859..11488)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatctagag gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttcgat ctagaggatc catggtgagc aagggcgagg agctgttcac
     2941 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt
     3001 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3061 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca
     3121 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3181 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3241 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3301 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3361 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3421 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3481 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3541 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3601 cactcacggc atggacgagc tgtacaagta aagcggcccg agctcgaatt tccccgatcg
     3661 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3721 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     3781 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     3841 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     3901 actagatcgg gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg
     3961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4021 gcctggggtg cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc
     4081 tattaattcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     4141 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     4201 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     4261 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     4321 ttattgccaa atgtttgaac gatcggggaa attcggggga tcgatcccgc gaactgtgga
     4381 cgagaactgt gccaccaagc gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc
     4441 gagttgctgg ctgaggcgtt ctcgaaatca gctcttgttc ggtcggcatc tactctattc
     4501 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     4561 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     4621 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     4681 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     4741 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     4801 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     4861 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     4921 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     4981 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     5041 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     5101 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     5161 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     5221 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaatgcccc
     5281 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     5341 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     5401 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     5461 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     5521 atcttattgc cccccgggat cagatctgga ttgagagtga atatgagact ctaattggat
     5581 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     5641 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     5701 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     5761 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     5821 ctccgctcat gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     5881 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     5941 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     6001 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     6061 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     6121 cttgcgccct gagtgcttgc ggcagcgtga agctgatccg tcgagattaa tagaaataaa
     6181 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     6241 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     6301 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     6361 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     6421 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     6481 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     6541 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     6601 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     6661 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     6721 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     6781 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     6841 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     6901 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     6961 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     7021 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     7081 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     7141 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     7201 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     7261 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     7321 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     7381 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     7441 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     7501 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     7561 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     7621 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     7681 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     7741 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7981 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8461 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8521 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8581 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     8641 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     8701 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     8761 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     8821 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     8881 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     8941 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     9001 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     9061 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     9121 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     9181 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     9241 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     9301 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     9361 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     9421 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     9481 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     9541 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     9601 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     9661 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     9721 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     9781 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     9841 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     9901 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     9961 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
    10021 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
    10081 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
    10141 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
    10201 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
    10261 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
    10321 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
    10381 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
    10441 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
    10501 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
    10561 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
    10621 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
    10681 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
    10741 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
    10801 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
    10861 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
    10921 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
    10981 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
    11041 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
    11101 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
    11161 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
    11221 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
    11281 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
    11341 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    11401 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    11461 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    11521 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    11581 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    11641 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    11701 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    11761 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    11821 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    11881 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    11941 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    12001 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    12061 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    12121 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    12181 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    12241 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    12301 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    12361 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    12421 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    12481 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    12541 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    12601 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    12661 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    12721 cgaacggata aacct
//
