LOCUS       sequence-141523-       12728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            sGFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB506
FEATURES             Location/Qualifiers
     source          1..12728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1178..1180,1181..1183,1184..1891)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(1223..1244)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1895..2019
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2056..2061,2062..2079,2080..2086)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2140..2799
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2206..2225)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3141..3446
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3325..3344
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3487..3611)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3649..3901
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3935..3951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3935..3951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3948..3970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3959..3975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3983..3989,3990..4007,4008..4013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4028..4049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4085..4337)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4488..5513)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5565..5744)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6250..6274
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6795..7586
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7832..8420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8321..8340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8574..8591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8606..8746
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8672..8691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8832..8854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9090..9284
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9350..10423)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10852..11481)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact cacggcatgg acgagctgta catcacaagt ttgtacaaaa aagctgaacg
     1921 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1981 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
     2041 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     2101 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2161 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2221 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     2281 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     2341 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     2401 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     2461 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     2521 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     2581 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     2641 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     2701 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     2761 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     2821 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     2881 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga
     2941 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     3001 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     3061 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     3121 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     3181 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     3241 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     3301 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     3361 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     3421 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     3481 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     3541 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     3601 tacaaagtgg tgatgtacaa gtaaagcggc ccgagctcga atttccccga tcgttcaaac
     3661 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3721 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3781 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3841 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3901 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4021 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctattaat
     4081 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     4141 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     4201 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     4261 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     4321 caaatgtttg aacgatcggg gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac
     4381 tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg cgcgagttgc
     4441 tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta ttcctttgcc
     4501 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     4561 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     4621 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     4681 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
     4741 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     4801 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     4861 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     4921 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     4981 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     5041 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
     5101 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
     5161 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     5221 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaatgc cccaatgtca
     5281 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     5341 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     5401 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     5461 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcttat
     5521 tgccccccgg gatcagatct ggattgagag tgaatatgag actctaattg gataccgagg
     5581 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
     5641 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
     5701 ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
     5761 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
     5821 catgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     5881 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     5941 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     6001 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     6061 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     6121 cctgagtgct tgcggcagcg tgaagctgat ccgtcgagat taatagaaat aaattcagcc
     6181 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     6241 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     6301 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     6361 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     6421 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     6481 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     6541 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     6601 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     6661 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     6721 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     6781 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     6841 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     6901 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     6961 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     7021 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     7081 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     7141 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     7201 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     7261 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     7321 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     7381 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     7441 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     7501 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     7561 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     7621 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     7681 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     7741 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8521 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8581 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8641 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8701 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8761 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8821 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8881 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     8941 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     9001 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     9061 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     9121 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     9181 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     9241 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     9301 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     9361 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     9421 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     9481 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     9541 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9601 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9661 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9721 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9781 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9841 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9901 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     9961 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
    10021 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
    10081 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
    10141 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
    10201 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
    10261 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
    10321 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
    10381 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
    10441 gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc
    10501 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
    10561 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    10621 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    10681 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    10741 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    10801 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    10861 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    10921 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    10981 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    11041 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    11101 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    11161 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    11221 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    11281 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    11341 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    11401 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    11461 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    11521 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    11581 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    11641 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    11701 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    11761 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    11821 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    11881 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    11941 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    12001 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    12061 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    12121 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    12181 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    12241 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    12301 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    12361 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    12421 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    12481 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    12541 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    12601 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    12661 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    12721 ataaacct
//
