LOCUS       sequence-141532-       12128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            3xHA (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB515
FEATURES             Location/Qualifiers
     source          1..12128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1196..1285
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    1307..1431
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1468..1473,1474..1491,1492..1498)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1552..2211
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1618..1637)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2553..2858
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2737..2756
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2899..3023)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3049..3301
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3335..3351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3335..3351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3348..3370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3359..3375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3383..3389,3390..3407,3408..3413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3428..3449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3485..3737)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3888..4913)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4965..5144)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5650..5674
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6195..6986
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7232..7820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7721..7740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7974..7991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8006..8146
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8072..8091)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8232..8254
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8490..8684
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8750..9823)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10252..10881)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaaca tcttttaccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     1261 tccatatgac gttccagatt acgctgctca gtgcagcgct gttatcacaa gtttgtacaa
     1321 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     1381 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct
     1441 cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1501 tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa aatggagaaa
     1561 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1621 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1681 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1741 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1801 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1861 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1921 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1981 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2041 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2101 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
     2161 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga
     2221 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2281 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc
     2341 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2401 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2461 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2521 ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa
     2581 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2641 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2701 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2761 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2821 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2881 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2941 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     3001 tcagctttct tgtacaaagt ggtgataacc tagagctcga atttccccga tcgttcaaac
     3061 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3121 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3181 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3241 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3301 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3421 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctattaat
     3481 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     3541 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     3601 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     3661 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     3721 caaatgtttg aacgatcggg gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac
     3781 tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg cgcgagttgc
     3841 tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta ttcctttgcc
     3901 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
     3961 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
     4021 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
     4081 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
     4141 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     4201 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
     4261 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
     4321 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
     4381 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
     4441 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
     4501 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
     4561 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
     4621 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaatgc cccaatgtca
     4681 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
     4741 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
     4801 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
     4861 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcttat
     4921 tgccccccgg gatcagatct ggattgagag tgaatatgag actctaattg gataccgagg
     4981 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
     5041 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
     5101 ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
     5161 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
     5221 catgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     5281 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     5341 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     5401 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     5461 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     5521 cctgagtgct tgcggcagcg tgaagctgat ccgtcgagat taatagaaat aaattcagcc
     5581 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     5641 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     5701 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     5761 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     5821 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     5881 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     5941 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     6001 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     6061 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     6121 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     6181 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     6241 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     6301 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     6361 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     6421 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     6481 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     6541 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     6601 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     6661 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     6721 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     6781 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     6841 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     6901 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     6961 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     7021 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     7081 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     7141 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7981 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8041 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8101 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8161 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8221 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8281 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     8341 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     8401 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     8461 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     8521 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     8581 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     8641 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     8701 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     8761 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     8821 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     8881 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     8941 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9001 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9061 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9121 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9181 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9241 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9301 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     9361 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     9421 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     9481 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     9541 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     9601 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     9661 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     9721 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     9781 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     9841 gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc
     9901 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     9961 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    10021 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    10081 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    10141 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    10201 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    10261 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    10321 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    10381 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    10441 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    10501 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    10561 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    10621 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    10681 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    10741 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    10801 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    10861 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    10921 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    10981 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    11041 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    11101 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    11161 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    11221 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    11281 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    11341 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    11401 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    11461 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    11521 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    11581 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    11641 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    11701 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    11761 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    11821 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    11881 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    11941 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    12001 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    12061 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    12121 ataaacct
//
