LOCUS       sequence-141535-       12179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            4xMyc (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB518
FEATURES             Location/Qualifiers
     source          1..12179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1193..1222
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1229..1258
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1274..1303
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1310..1339
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1358..1482
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1519..1524,1525..1542,1543..1549)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1603..2262
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1669..1688)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2604..2909
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2788..2807
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2950..3074)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3100..3352
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3386..3402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3386..3402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3399..3421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3410..3426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3434..3440,3441..3458,3459..3464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3479..3500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3536..3788)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3939..4964)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5016..5195)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5701..5725
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6246..7037
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7283..7871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7772..7791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8025..8042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8057..8197
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8123..8142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8283..8305
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8541..8735
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8801..9874)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10303..10932)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaacg gtgaacaaaa
     1201 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     1261 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     1321 aatctccgag gaagacttga acggtagcgc tgttatcaca agtttgtaca aaaaagctga
     1381 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     1441 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
     1501 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1561 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1621 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1681 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1741 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1801 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1861 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1921 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1981 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2041 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2101 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2161 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     2221 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     2281 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2341 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     2401 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2461 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2521 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2581 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2641 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2701 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2761 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2821 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2881 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2941 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3001 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3061 ttgtacaaag tggtgataac ctagagctcg aatttccccg atcgttcaaa catttggcaa
     3121 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3181 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3241 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3301 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3361 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3481 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3541 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3601 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     3661 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     3721 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     3781 gaacgatcgg ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc
     3841 aagcgtaagg ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg
     3901 cgttctcgaa atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga
     3961 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     4021 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     4081 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     4141 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     4201 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     4261 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     4321 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     4381 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     4441 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     4501 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     4561 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     4621 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     4681 gcacggcggg agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc
     4741 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     4801 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     4861 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     4921 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctta ttgccccccg
     4981 ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     5041 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     5101 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     5161 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     5221 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt
     5281 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     5341 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
     5401 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     5461 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     5521 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc
     5581 ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc
     5641 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     5701 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     5761 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     5821 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     5881 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     5941 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6001 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6061 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6121 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6181 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6241 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6301 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6361 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6421 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6481 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6541 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6601 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6661 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     6721 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     6781 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     6841 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     6901 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     6961 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7021 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7081 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     7141 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7201 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7921 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7981 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8041 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8101 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8161 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8221 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8281 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     8341 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     8401 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8461 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8521 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8581 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     8641 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     8701 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     8761 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     8821 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     8881 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     8941 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9001 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9061 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9121 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9181 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9241 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     9301 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     9361 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9421 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9481 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9541 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9601 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     9661 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     9721 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     9781 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     9841 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     9901 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     9961 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10021 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10081 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10141 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    10201 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    10261 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    10321 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    10381 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10441 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10501 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10561 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10621 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10681 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    10741 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    10801 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    10861 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    10921 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    10981 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11041 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11101 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    11161 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    11221 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    11281 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    11341 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    11401 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11461 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11521 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11581 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11641 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    11701 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    11761 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    11821 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    11881 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    11941 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12001 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12061 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12121 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
