LOCUS       sequence-141537-       12408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            10xMyc (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB520
FEATURES             Location/Qualifiers
     source          1..12408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2921..2950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2957..2986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3002..3031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3038..3067
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3074..3103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3119..3148
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3155..3184
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3191..3220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3236..3265
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3272..3301
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3329..3581
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3615..3631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3615..3631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3628..3650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3639..3655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3663..3669,3670..3687,3688..3693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3708..3729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3765..4017)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4168..5193)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5245..5424)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5930..5954
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6475..7266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7512..8100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8001..8020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8254..8271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8286..8426
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8352..8371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8512..8534
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8770..8964
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9030..10103)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10532..11161)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacggt gaacaaaagc taatctccga
     2941 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg
     3001 tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga
     3061 agacttgaac ggtgaacaaa aattaatctc agaagaagac ttgaacggac tcgacggtga
     3121 acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct ccgaggaaga
     3181 cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg acggtgaaca
     3241 aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg aggaagactt
     3301 gaacggtgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     3361 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     3421 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     3481 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     3541 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc
     3601 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3721 ctcacattag gctgaattag gcgcgcctat ttctattaat tcccgatcta gtaacataga
     3781 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
     3841 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
     3901 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
     3961 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
     4021 gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac tgtgccacca agcgtaaggc
     4081 cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa
     4141 tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
     4201 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     4261 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     4321 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     4381 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
     4441 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
     4501 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     4561 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     4621 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     4681 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     4741 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     4801 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     4861 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     4921 gatgcaatag gtcaggctct cgctgaatgc cccaatgtca agcacttccg gaatcgggag
     4981 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
     5041 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
     5101 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
     5161 gacagacgtc gcggtgagtt caggcttttt catatcttat tgccccccgg gatcagatct
     5221 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     5281 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     5341 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
     5401 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
     5461 atcggcgggg gtcataacgt gactccctta attctccgct catgatcttg atcccctgcg
     5521 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     5581 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
     5641 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
     5701 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
     5761 ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg
     5821 tgaagctgat ccgtcgagat taatagaaat aaattcagcc taattcggcg ttaattcagt
     5881 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     5941 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     6001 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     6061 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     6121 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     6181 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     6241 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     6301 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     6361 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     6421 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     6481 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     6541 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     6601 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     6661 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     6721 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     6781 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     6841 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     6901 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     6961 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     7021 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     7081 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     7141 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     7201 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     7261 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     7321 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     7381 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
     7441 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7501 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7561 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7621 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7681 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7741 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7801 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7861 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7921 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7981 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8041 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8101 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8161 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8221 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8281 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     8341 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     8401 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     8461 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8521 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     8581 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     8641 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     8701 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     8761 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     8821 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     8881 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     8941 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     9001 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     9061 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     9121 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     9181 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     9241 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     9301 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     9361 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     9421 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     9481 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     9541 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     9601 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     9661 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     9721 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     9781 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     9841 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     9901 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     9961 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
    10021 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
    10081 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
    10141 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
    10201 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    10261 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    10321 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    10381 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    10441 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    10501 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    10561 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    10621 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    10681 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    10741 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    10801 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    10861 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    10921 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    10981 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    11041 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    11101 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    11161 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    11221 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    11281 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    11341 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    11401 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    11461 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    11521 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    11581 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    11641 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    11701 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    11761 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    11821 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    11881 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    11941 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    12001 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    12061 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    12121 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    12181 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    12241 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    12301 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    12361 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaacct
//
