LOCUS       sequence-141541-       12686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            GST (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB524
FEATURES             Location/Qualifiers
     source          1..12686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1831
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1789..1811
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1838..1855
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    1865..1989
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2026..2031,2032..2049,2050..2056)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2110..2769
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2176..2195)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3111..3416
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3295..3314
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3457..3581)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3607..3859
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3893..3909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3893..3909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3906..3928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3917..3933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3941..3947,3948..3965,3966..3971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3986..4007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4043..4295)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4446..5471)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5523..5702)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6208..6232
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6753..7544
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7790..8378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8279..8298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8532..8549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8564..8704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8630..8649)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8790..8812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9048..9242
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9308..10381)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10810..11439)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg tcccctatac taggttattg
     1201 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
     1261 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
     1321 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
     1381 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
     1441 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
     1501 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
     1561 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
     1621 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
     1681 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
     1741 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
     1801 cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatccgctgt
     1861 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1921 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1981 gtcactatgg cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct
     2041 tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag
     2101 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     2161 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     2221 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     2281 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     2341 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2401 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2461 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2521 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     2581 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2641 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     2701 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2761 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     2821 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     2881 ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     2941 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     3001 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     3061 gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta
     3121 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     3181 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     3241 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     3301 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     3361 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     3421 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     3481 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3541 tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataaccta gagctcgaat
     3601 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3661 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3721 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3781 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3841 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3961 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4021 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     4081 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     4141 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     4201 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     4261 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcggggg atcgatcccg
     4321 cgaactgtgg acgagaactg tgccaccaag cgtaaggccg ttctctcgca tttgccttgc
     4381 taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat
     4441 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     4501 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     4561 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     4621 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     4681 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     4741 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     4801 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     4861 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     4921 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     4981 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     5041 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     5101 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     5161 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     5221 ctgaatgccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     5281 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     5341 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     5401 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     5461 ggctttttca tatcttattg ccccccggga tcagatctgg attgagagtg aatatgagac
     5521 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
     5581 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
     5641 tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt
     5701 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
     5761 ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct tggcggcaag
     5821 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     5881 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     5941 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     6001 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     6061 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta
     6121 atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     6181 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     6241 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     6301 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     6361 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     6421 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac
     6481 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     6541 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     6601 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     6661 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     6721 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat
     6781 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     6841 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     6901 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     6961 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     7021 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     7081 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     7141 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     7201 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     7261 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     7321 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     7381 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     7441 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     7501 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt
     7561 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
     7621 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     7681 aataagccct acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt
     7741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7921 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8581 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     8641 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     8701 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8821 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8881 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8941 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     9001 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     9061 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     9121 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     9181 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     9241 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     9301 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     9361 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     9421 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     9481 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     9541 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     9601 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     9661 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9721 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9781 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9841 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9901 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9961 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    10021 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    10081 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    10141 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    10201 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    10261 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    10321 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    10381 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc
    10441 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    10501 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    10561 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    10621 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    10681 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    10741 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10801 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    10861 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    10921 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    10981 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    11041 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    11101 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    11161 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    11221 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    11281 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    11341 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    11401 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    11461 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    11521 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    11581 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    11641 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    11701 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    11761 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    11821 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    11881 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    11941 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    12001 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    12061 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    12121 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    12181 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    12241 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    12301 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    12361 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    12421 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    12481 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    12541 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    12601 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    12661 atacaaatgg acgaacggat aaacct
//
