LOCUS       sequence-141545-       11696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            TAP (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB528
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..2127
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2155..2175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2215..2388
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2389..2562
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      2617..2869
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2903..2919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2903..2919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2916..2938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2927..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2951..2957,2958..2975,2976..2981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2996..3017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3053..3305)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3456..4481)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4533..4712)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5218..5242
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5763..6554
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6800..7388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7289..7308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7542..7559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7574..7714
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7640..7659)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7800..7822
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8058..8252
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8318..9391)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9820..10449)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca agagaagatg gaaaaagaat ttcatagccg tctcagcagc caaccgcttt
     2101 aagaaaatct catcctccgg ggcacttgat tatgatattc caactactgc tagcgagaat
     2161 ttgtattttc agggtgagct caaaaccgcg gctcttgcgc aacacgatga agccgtggac
     2221 aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta
     2281 aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct
     2341 aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt agacaacaaa
     2401 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
     2461 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
     2521 ttagcagaag ctaaaaagct aaatggtgct caggcgccga aagtagacgc gaattccgcg
     2581 gggaagtcaa cctgagctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     2641 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     2701 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2761 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2821 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     2881 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3001 tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt
     3061 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     3121 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     3181 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     3241 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     3301 cgatcgggga aattcggggg atcgatcccg cgaactgtgg acgagaactg tgccaccaag
     3361 cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt
     3421 tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg
     3481 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     3541 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     3601 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     3661 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
     3721 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     3781 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     3841 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     3901 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     3961 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     4021 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     4081 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
     4141 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     4201 cggcgggaga tgcaataggt caggctctcg ctgaatgccc caatgtcaag cacttccgga
     4261 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     4321 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     4381 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     4441 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcttattg ccccccggga
     4501 tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     4561 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     4621 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     4681 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     4741 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat
     4801 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     4861 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     4921 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     4981 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     5041 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg
     5101 cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt
     5161 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     5221 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     5281 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     5341 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     5401 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     5461 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     5521 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     5581 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     5641 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     5701 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     5761 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     5821 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     5881 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     5941 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     6001 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     6061 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     6121 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     6181 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     6241 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     6301 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     6361 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     6421 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     6481 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     6541 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     6601 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     6661 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     6721 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7441 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7501 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7561 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7621 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     7681 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     7741 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7801 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     7861 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     7921 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     7981 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     8041 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     8101 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     8161 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     8221 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     8281 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     8341 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     8401 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     8461 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     8521 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     8581 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     8641 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     8701 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     8761 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     8821 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     8881 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     8941 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     9001 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     9061 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     9121 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     9181 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     9241 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     9301 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     9361 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     9421 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     9481 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     9541 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     9601 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     9661 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     9721 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     9781 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     9841 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     9901 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     9961 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
    10021 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
    10081 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
    10141 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
    10201 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
    10261 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
    10321 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
    10381 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
    10441 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
    10501 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
    10561 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
    10621 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
    10681 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
    10741 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
    10801 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
    10861 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
    10921 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
    10981 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
    11041 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
    11101 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
    11161 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
    11221 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
    11281 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
    11341 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
    11401 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
    11461 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
    11521 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
    11581 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
    11641 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
