LOCUS       sequence-141547-       12977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            GUS (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB533
FEATURES             Location/Qualifiers
     source          1..12977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(499..504,505..522,523..529)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             583..1242
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(649..668)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1584..1889
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1768..1787
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1930..2054)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..3876
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3898..4150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4184..4200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4184..4200)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4197..4219)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4208..4224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4232..4238,4239..4256,4257..4262))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4277..4298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4334..4586)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4737..5762)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5814..5993)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6499..6523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7044..7835
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8081..8669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8570..8589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8823..8840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8855..8995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8921..8940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9081..9103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9339..9533
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9599..10672)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11101..11730)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatgtta cgtcctgtag aaaccccaac ccgtgaaatc
     2101 aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag
     2161 cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac
     2221 gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa
     2281 gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact
     2341 cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg
     2401 ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt
     2461 tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa
     2521 aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc
     2581 agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat
     2641 gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc
     2701 agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg
     2761 actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg
     2821 tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg
     2881 tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc
     2941 tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg
     3001 cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct
     3061 tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact
     3121 gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa
     3181 gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt
     3241 aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac
     3301 gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg
     3361 cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac
     3421 accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat
     3481 gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg
     3541 caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg
     3601 ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg
     3661 tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc
     3721 gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact
     3781 cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac
     3841 ttcggtgaaa aaccgcagca gggaggcaaa caatgagctt agagctcgaa tttccccgat
     3901 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     3961 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     4021 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     4081 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     4141 ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag ctgtttcctg
     4201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4261 aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg cgcgcctatt
     4321 tctattaatt cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     4381 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     4441 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     4501 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     4561 ctttattgcc aaatgtttga acgatcgggg aaattcgggg gatcgatccc gcgaactgtg
     4621 gacgagaact gtgccaccaa gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc
     4681 gcgagttgct ggctgaggcg ttctcgaaat cagctcttgt tcggtcggca tctactctat
     4741 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     4801 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     4861 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     4921 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     4981 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     5041 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     5101 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     5161 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     5221 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     5281 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     5341 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     5401 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     5461 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaatgcc
     5521 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     5581 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     5641 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     5701 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     5761 atatcttatt gccccccggg atcagatctg gattgagagt gaatatgaga ctctaattgg
     5821 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     5881 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     5941 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
     6001 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     6061 ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     6121 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     6181 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     6241 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     6301 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
     6361 cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt aatagaaata
     6421 aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     6481 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     6541 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     6601 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     6661 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     6721 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     6781 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     6841 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     6901 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     6961 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     7021 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     7081 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     7141 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     7201 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     7261 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     7321 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     7381 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     7441 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     7501 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     7561 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     7621 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     7681 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     7741 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     7801 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     7861 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     7921 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     7981 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8221 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8821 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     8881 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     8941 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     9001 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     9061 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     9121 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     9181 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     9241 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     9301 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     9361 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     9421 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     9481 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     9541 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     9601 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     9661 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     9721 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     9781 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     9841 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     9901 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     9961 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
    10021 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
    10081 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
    10141 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
    10201 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
    10261 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
    10321 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
    10381 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
    10441 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
    10501 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
    10561 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
    10621 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
    10681 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
    10741 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
    10801 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
    10861 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
    10921 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
    10981 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    11041 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
    11101 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
    11161 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
    11221 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
    11281 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
    11341 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
    11401 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
    11461 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    11521 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    11581 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    11641 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    11701 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    11761 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    11821 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    11881 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    11941 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    12001 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    12061 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    12121 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    12181 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    12241 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    12301 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    12361 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    12421 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    12481 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    12541 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    12601 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    12661 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    12721 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    12781 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    12841 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    12901 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    12961 gacgaacgga taaacct
//
