LOCUS       sequence-141560-       12683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            mRFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB555
FEATURES             Location/Qualifiers
     source          1..12683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1852
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1178..1852
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     1576..1595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1770..1793
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    1862..1986
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2023..2028,2029..2046,2047..2053)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2107..2766
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2173..2192)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3108..3413
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3292..3311
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3454..3578)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3604..3856
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3890..3906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3890..3906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3903..3925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3914..3930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3938..3944,3945..3962,3963..3968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3983..4004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4040..4292)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4443..5468)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5520..5699)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6205..6229
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6750..7541
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7787..8375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8276..8295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8529..8546
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8561..8701
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8627..8646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8787..8809
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9045..9239
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9305..10378)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10807..11436)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gcctcctccg aggacgtcat
     1201 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     1261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     1321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
     1381 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     1441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     1501 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     1561 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     1621 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
     1681 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     1741 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     1801 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccgctgttat
     1861 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     1921 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     1981 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2041 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
     2101 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2161 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2221 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2281 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2341 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2401 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2461 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2521 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2581 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2641 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     2701 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2761 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     2821 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2881 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2941 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     3001 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     3061 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     3121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     3181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     3421 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     3481 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3541 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taacctagag ctcgaatttc
     3601 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     3781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     3841 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     3901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4021 cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     4081 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     4141 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     4201 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     4261 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgggggatc gatcccgcga
     4321 actgtggacg agaactgtgc caccaagcgt aaggccgttc tctcgcattt gccttgctag
     4381 gctcgcgcga gttgctggct gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta
     4441 ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     4501 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     4561 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     4621 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     4681 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     4741 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     4801 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     4861 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     4921 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     4981 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     5041 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     5101 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     5161 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     5221 aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     5281 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     5341 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     5401 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     5461 tttttcatat cttattgccc cccgggatca gatctggatt gagagtgaat atgagactct
     5521 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     5581 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     5641 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     5701 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     5761 ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     5821 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     5881 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     5941 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     6001 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     6061 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata
     6121 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     6181 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     6241 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     6301 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     6361 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6421 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6481 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6541 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6601 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6661 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6721 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6781 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6841 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6901 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     6961 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     7021 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     7081 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     7141 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     7201 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7261 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7321 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7381 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7441 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7501 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7561 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7621 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7681 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7741 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7801 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7861 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7921 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7981 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8041 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8101 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8161 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8221 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8281 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8341 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8401 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8461 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8521 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8581 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8641 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8701 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8761 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8821 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8881 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8941 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     9001 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     9061 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     9121 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     9181 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     9241 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     9301 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     9361 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9421 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9481 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9541 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9601 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9661 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9721 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9781 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9841 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9901 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9961 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
    10021 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
    10081 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
    10141 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
    10201 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    10261 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    10321 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
    10381 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    10441 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10501 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10561 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10621 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10681 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10741 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10801 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10861 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10921 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    10981 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    11041 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    11101 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    11161 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    11221 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    11281 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    11341 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    11401 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    11461 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11521 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11581 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11641 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11701 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11761 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11821 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11881 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    11941 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    12001 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    12061 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    12121 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    12181 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    12241 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    12301 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    12361 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    12421 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12481 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12541 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12601 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12661 caaatggacg aacggataaa cct
//
