LOCUS       sequence-141564-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified plasmid with a different backbone that yields higher titers
            of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines 
            fused to N-terminus of AAV9.47 VP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Choudhury SR, Harris AF, Cabral DJ, Keeler AM, Sapp E, Ferreira JS, 
            Gray-Edwards HL, Johnson JA, Johnson AK, Su Q, Stoica L, DiFiglia M,
            Aronin N, Martin DR, Gao G, Sena-Esteves M
  TITLE     Widespread Central Nervous System Gene Transfer and Silencing After 
            Systemic Delivery of Novel AAV-AS Vector.
  JOURNAL   Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2015.231.
  PUBMED    26708003
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2015 Dec 28. doi: 10.1038/mt.2015.231."
COMMENT     Sequence Label: pGG-9.47_VP2_AS #2562
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     26..42
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attgggtacc gggccccccc tcgatcgagg tcgacggtat cgggggagct cgcagggtct
       61 ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg
      121 attaaggtcc ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac
      181 tgggtggccg agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt
      241 gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgc
      301 cgtgtgagta aggccccgga ggctcttttc tttgtgcaat ttgagaaggg agagagctac
      361 ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc
      421 ctgagtcaga ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg
      481 ccaaactggt tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa caaggtggtg
      541 gatgagtgct acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg
      601 tggactaata tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg
      661 gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat
      721 cccaattctg atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc
      781 gggtggctcg tggacaagg
//
