LOCUS       sequence-141575-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human RPN13 PRU domain in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GST-PP-RPN13 (19-132)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..696
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     654..676
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             703..726
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ctttaagaag gagatataca tatgggaatt gatcccttca ccatgtcccc tatactaggt
       61 tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata tcttgaagaa
      121 aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt
      181 gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca
      241 cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca
      301 aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt
      361 tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta
      421 cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat
      481 catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac
      541 ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc
      601 ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg
      661 caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg atctggaagt tctgttccag
      721 gggccgtcca acaagtactt ggtggagttt cgggcgggaa agatgtccct gaaggggacc
      781 accgtgactc cggataagcg gaaagggctg gtgtacatt
//
