LOCUS       sequence-141576-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human RPN13 PRU domain in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GST-PP-RPN13 (19-132)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(385..408)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(435..457)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctgag ctcgcccttt tacggggggt tgttcagata
       61 ctcgttgact ttccggcaat gctcctcatc ctggtctgtc ttgggttcct gcatccagaa
      121 gaaaagccgc ttggaccctg ccttgaactt cagcacgtag accctcccgc tggggcactg
      181 cggcacccgc ttgaactcac agtcgtcagg gaagatgatc aagtcgtctt ccacgttccc
      241 ggacgtcctg tccttccagc agaagtgaat aagcgagtcg tccgtctgct gaatgtacac
      301 cagccctttc cgcttatccg gagtcacggt ggtccccttc agggacatct ttcccgcccg
      361 aaactccacc aagtacttgt tggacggccc ctggaacaga acttccagat ccgattttgg
      421 aggatggtcg ccaccaccaa acgtggcttg ccagccctgc aaaggccatg ctatatactt
      481 gctggatttc aagtacttat caatttgtgg gatagcttca atacgttttt taaaacaaac
      541 taattttggg aacgcatcca ggcacattgg gtccatgtat aaaacaacat caagagcgtc
      601 atacaacatg aagtcaggat gggttacatg atcaccattt aaatatgttt tatgacataa
      661 acgatcttcg aacattttca gcatttcagg tagcttgcta agaaaatcaa ctttgagagt
      721 ttcaaagtct ttactatatg caattctcga aacaccgtat ctaatatcca aaaccgctcc
      781 ttcaagcatt gaaatctctg cacgctcttt tggacaacca cccaacatgt tgtgcttgtc
      841 agctatataa
//
