LOCUS       sequence-141587-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SERCA in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Nakamoto lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAGHis10pFastBacHTA/Serca1
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             109..138
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
       61 cggtccgaaa ccatgtcgta ctacgattac aaggatgacg acgataagca tcaccatcac
      121 catcaccatc accatcacga ttacgatatc ccaacgaccg aaaacctgta ttttcagggc
      181 gccatggagg ccgcgcactc caagagcaca gaggaatgtc tggcctattt tggggtgagc
      241 gagaccacag gcctcacccc ggaccaagtg aagcgacatc tagagaaata cggccacaat
      301 gagcttcctg ctgaggaagg gaaatccctg tgggagctgg tgatagagca gtttgaagac
      361 ctcctggtgc ggattcttct gctggccgcc tgcatctcct ttgtgctggc ctggtttgaa
      421 gaaggggaag agaccatcac tgccttcgtt gagccctttg tcatcctcct gatcctcatt
      481 gccaatgcca tcgtgggagt ttggcaggag cggaacgctg agaacgccat agaggcgctg
      541 aaggaatatg agcccgagat ggggaaggtg taccgggctg accgcaagtc agtgcaaagg
      601 atcaaggctc gggacatcgt ccccggggac atcgtggagg tggcggttgg ggacaaagtc
      661 cctgcagaca tccgcatcct gtctatcaag tccaccaccc tccgcgtgga ccagtccatc
      721 ctgacaggcg agtccgtgtc cgtcatcaag cacacggagc cagtccctga cccgcgggct
      781 gtcaaccagg acaagaagaa catgcttttc tcgggtacca acatcgcggc cggcaag
//
