LOCUS       sequence-141589-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP-PP-RPN2 (940-953) fusion in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GFP-PP-RPN2 (940-953)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..109)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(152..173)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     501..520
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     760..781
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctttcgggct ttgtagcagc cggaatctga gctcgccctt ttaatcatca atatactcaa
       61 atggttctgg gggttctggc tcctgcggcc cctggaacag aacttccagc ttatacagct
      121 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      181 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
      241 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      301 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
      361 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      421 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      481 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      541 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      601 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
      661 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
      721 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      781 ggatgggcac caccccggtg aacagctcct cgcccttt
//
