LOCUS       sequence-141592-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP-PP-RPN2 (918-947) fusion in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GFP-PP-RPN2 (918-947)
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..156)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(199..220)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     548..567
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctgag ctcgcccttt tatggttctg ggggttctgg
       61 ctcttgttcc tcctcctcga tttttgggcc atgtgctgcc acaggttcca ccagctcctc
      121 aatgtcttca ctcggcccct ggaacagaac ttccagctta tacagctcgt ccatgccgag
      181 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      241 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      301 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      361 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
      421 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      481 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      541 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      601 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tgg
//
