LOCUS       sequence-141596-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SERCA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     Nakamoto lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1/HisA/Serca1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..168
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(172..180,181..195)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagctta ccatgggggg ttctcatcat
      121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      181 gacgatgacg ataaggtacc taggatccag tgtggtggaa ttcaggaggg cgccatggag
      241 gccgcgcact ccaagagcac agaggaatgt ctggcctatt ttggggtgag cgagaccaca
      301 ggcctcaccc cggaccaagt gaagcgacat ctagagaaat acggccacaa tgagcttcct
      361 gctgaggaag ggaaatccct gtgggagctg gtgatagagc agtttgaaga cctcctggtg
      421 cggattcttc tgctggccgc ctgcatctcc tttgtgctgg cctggtttga agaaggggaa
      481 gagaccatca ctgccttcgt tgagcccttt gtcatcctcc tgatcctcat tgccaatgcc
      541 atcgtgggag tttggcagga gcggaacgct gagaacgcca tagaggcgct gaaggaatat
      601 gagcccgaga tggggaaggt gtaccgggct gaccgcaagt cagtgcaaag gatcaaggct
      661 cgggacatcg tccccgggga catcgtggag gtggcggttg gggacaaagt ccctgcagac
      721 atccgcatcc tgtctatcaa gtccaccacc ctccgcgtgg accagtccat cctgacaggc
      781 gagtccgtgt ccgtcatcaa gcacacggag ccagtccctg acccgcgggc tgtcaaccag
      841 gaca
//
