LOCUS       sequence-141597-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GFP-PP-RPN2 (797-953)
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(507..530)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(573..594)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctttgttagc agccggatct gagctcgccc ttttaatcat caatatactc aaatggttct
       61 gggggttctg gctcttgttc ctcctcctcg atttttgggc catgtgctgc cacaggttcc
      121 accagctcct caatgtcttc actggtatcc ttcagaatga tgatgcctcc aatagagagt
      181 ggtttgaaag gctggtatct acaggtctcc ggcatggtta ggaccttaag ctgggcaggc
      241 ataactcggg ctgggttatc caataactgg aagtttggct caggttcttt tttcttctct
      301 ttttcctcct ttttctctgc ctcatccact tccattttct cctcctcctt tttttccttt
      361 tctttttcct tctttttagc cttggcagtt atagataata cagcagtaga aaccttttcc
      421 ttttcttttt cttttggtac ttccagaggg gcaggatatg caaatgtgga tggtttacag
      481 ttcgatttat actgaacttt cggcatcggc ccctggaaca gaacttccag cttatacagc
      541 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      601 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
      661 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      721 ctgccgt
//
