LOCUS       sequence-141602-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R132 mutant IDH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, 
            Peng Y, Ding J, Lei Q, Guan KL, Xiong Y
  TITLE     Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic 
            activity and induce HIF-1alpha.
  JOURNAL   Science. 2009 Apr 10;324(5924):261-5. doi: 10.1126/science.1170944.
  PUBMED    19359588
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-04-10- 10"; volume: "324"; 
            issue: "5924"; pages: "261-5"
COMMENT     Sequence Label: pcDNA3-Flag-IDH1-R132H
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctttagcga ctctctggct actagagaac ccactgcctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagcgtttaa acgggccctc tagactcgag
      121 cggccgccac tgtgctggat atctgcagaa ttcatgtcca aaaaaatcag tggcggttct
      181 gtggtagaga tgcaaggaga tgaaatgaca cgaatcattt gggaattgat taaagagaaa
      241 ctcatttttc cctacgtgga attggatcta catagctatg atttaggcat agagaatcgt
      301 gatgccacca acgaccaagt caccaaggat gccgcagaag ctataaagaa gcataatgtt
      361 ggcgtcaaat gtgccactat cactcctgat gagaagaggg ttgaggagtt caagttgaaa
      421 caaatgtgga aatcaccaaa tggcaccata cgaaatattc tgggtggcac ggtcttcaga
      481 gaagccatta tctgcaaaaa tatcccccgg cttgtgagtg gatgggtaaa acctatcatc
      541 ataggtcatc atgcttatgg ggatcaatac agagcaactg attttgttgt tcctgggcct
      601 ggaaaagtag agataaccta cacaccaagt gacggaaccc aaaaggtgac atacctggta
      661 cataactttg aagaaggtgg tggtgttgcc atggggatgt ataatcaaga taagtcaatt
      721 gaagattttg cacacagttc cttccaaatg gctctgtcta agggttggcc tttgtatctg
      781 agcaccaaaa acactattct gaagaaatat gatgggcgtt ttaaagacat ctttcaggag
      841 atatatgaca agcagtacaa gtcccagttt gaaagctcaa aagatctggt atgagcatag
      901 gctcatcgac gacatggtgg cccaagctat gaaatcaga
//
