LOCUS       sequence-141604-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses R132 mutant IDH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, 
            Peng Y, Ding J, Lei Q, Guan KL, Xiong Y
  TITLE     Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic 
            activity and induce HIF-1alpha.
  JOURNAL   Science. 2009 Apr 10;324(5924):261-5. doi: 10.1126/science.1170944.
  PUBMED    19359588
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-04-10- 10"; volume: "324"; 
            issue: "5924"; pages: "261-5"
COMMENT     Sequence Label: pcDNA3-Flag-IDH1-R132H
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..21)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(37..66)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(80..103)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcaatgatga tgatgatgat ggtcgacggc gctattcaga tcctcttctg agatgagttt
       61 ttgttctaga aagcttctac ttatcgtcgt catccttgta atcgggatcc acaagtttgg
      121 cctgagctag tttgatcttc aagttttctc caagtttatc catgaactca aatgtattca
      181 agtagtcaga acgttgcaca ttgggtaaac ctttaatgca agcagccaag tccttggtca
      241 tgaagccagc ctcaattgtc tcaatagaga cttcttccaa agcatttgca aagaaggcaa
      301 gctctttatt gttatcaagc tttgctctgt gggctaaccc tctggtccag gcaaaaatgg
      361 aagcaatggg attggtggac gtctcctgtc ctttctggta catgcggtag tgtcgggtta
      421 cagtcccgtg ggcagcctct gcttctactg tcttgccatc tggacaaacc agcacgctgg
      481 tcatcatgcc gagagagcca tacccttggg ccacagagtc cgactgcacg tcaccatcat
      541 agtttttaca ggcccagatg aagcctccct ctgatttcat agcttgggcc accatgtcgt
      601 cgatgagcct atgctcatac cagatctttt gagcttcaaa ctgggacttg tactgcttgt
      661 catatatctc ctgaaagatg tctttaaaac gcccatcata tttcttcaga atagtgtttt
      721 tggtgctcag atacaaaggc caacccttag acagagccat ttggaaggaa ctgtgtgcaa
      781 aatcttcaat tgacttatct tgattataca tccccatggc aacaccacca ccttcttcaa
      841 agttatgtac c
//
