LOCUS       sequence-141609-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-Ago2 lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, 
            Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD
  TITLE     Alternative RISC assembly: binding and repression of microRNA-mRNA 
            duplexes by human Ago proteins.
  JOURNAL   RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012
            Sep 27.
  PUBMED    23019594
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.035675.112"; journalName: "RNA"; date: "2012-11"; 
            volume: "18"; issue: "11"; pages: "2041-55"
COMMENT     Sequence Label: pHAGE-eGFP-Ago2
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     269..290
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          354..557
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(377..397)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
       61 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
      121 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      181 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      241 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      301 accccggtga acagctcctc gcccttgctc accatgcggc cgcttcacga cacctgaaat
      361 ggaagaaaaa aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca
      421 aaaagggcaa attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc
      481 acccactcag tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac
      541 gtactccaaa agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag
      601 tcacatgaag cgacggctga ggacggaaag gcccttttcc tttgtgtggg tgactcaccc
      661 gcccgctctc ccgagcgccg cgtcctccat tttgagctcc ctgcagc
//
