LOCUS       sequence-141614-        5366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla control plasmid driven by the ubiquitin 63E promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pRL-ubi63E
FEATURES             Location/Qualifiers
     source          1..5366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2355..3290
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    3321..3442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3332..3351)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3386..3405
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3575..3679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3680..3748,3749..4540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3898..3917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4711..5299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5200..5219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggtgtcg ccggaacgca gcgacagaga
      361 ttccaatgtg tccgtatctt tcaggctttt gcccttcagt tccagacgaa gcgactggcg
      421 attcgcgtgt ggggtctgct tcagggtctt gtgaattagg gcgcgcagat cgccgatggg
      481 cgtggcgccg gagggcacct tcaccttgcc gtacggcttg ctgttcttcg cgttcaaaat
      541 ctccagctcc attttgcttt cggtgcgctt gcaatcagta ctgtccaaaa tcgaaaatcg
      601 ccgaaccgta gtgtgaccgt gcggggctct gcgaaaataa acttttttag gtatatggcc
      661 acacacgggg aaagcacagt ggattatatg ttttaatatt ataaatatgc aggttttcat
      721 tacttatcca gatgtaagcc cacttaaagc gatttaacaa ttatttgccg aaagagtata
      781 aacaaatttc acataaaaat ggattaagaa aagcttgtgt aagattatgc gcagcgttgc
      841 cagatagctc catttaaaac acttcaaaaa caataagttt agaaaatata tacataaata
      901 gcagtcgttg ccgcaacgct caacacatca cacttttaaa acacccttta cctacacaga
      961 attacttttt aaatttccag tcaagctgcg agtttcaaaa ttatagccgg tagagaagac
     1021 agtgctattt caaaagcaaa ctaacaaggg tcttaaattt caaaacacca atcctaacaa
     1081 gccttggact tttgtaagtt tagatcaaag gtggcattgc attcaatgtc atggtaagaa
     1141 gtaggtcgtc taggtagaaa tcctcattca gccggtcaag tcagtacgag aaaggtctca
     1201 atttgaaatt gtcttaaaaa tattttattg ttttgtactg tggtgagttt aaacgaaaaa
     1261 cacaaaaaaa aagtgataca cagaaatcat aaaaaatttt aatacaaggt attcgtacgt
     1321 atcaaaaaca tttcggcaca attttttttc tctgtactaa agtgttacga acactacggt
     1381 attttttagt gattttcaac ggacaccgaa ggtatataaa cagcgttcgc gaacggtcgc
     1441 cttcaaaacc aattgacatt tgcagcagca agtacaagta gaaagtaaag cgcaatcagc
     1501 gaaaaattta tacttaattg ttggtgatta aagtacaatt aaaagaacat tctcgaaagt
     1561 cacaagaaac gtaagttttt aactcgctgt taccaattag taataagagc aacaagacgt
     1621 tgagtaattt caagaaaaac tgcatttcaa ggtctttgtt cggccatttt ttttttattc
     1681 aacgctctac gtaattacaa aataagaaat tggcagccac gcatcttgtt ttcccaatga
     1741 attggcatca aaacgcaaac aaatctataa ataaaacttg cgtgttgatt ttcgccaaga
     1801 tttattggca aattgtgaaa ttcgcagtga cgcatttgaa aattcgagaa atcacgaacg
     1861 cactcgagca tttgtgtgca tgttattagt tagttgttta gttaattgaa gtattttacc
     1921 aacgaaatcc acttattttt agctgaaata gagtaggttg cttaaacaaa gccacgtctg
     1981 aaaatttctt attgcttgta gttgtgacgt caccatatac acacaaaata atgtgtatgc
     2041 atgcatttca gctgtgtata tatacatgca cacactcgca acacgaaaac gatgacgaag
     2101 caacggaaca aaggtttctc aactaccctt tgttccctgt ttcttcgctt tcctttgttc
     2161 caatattcgt agagggttaa taggggtttc tcaacaaagt tggcgtcgat aaataagttt
     2221 cccattttta ttccccagcc aggaagttag tttcaatagt tttgtaattt caacgaaact
     2281 catttgattt cgtactaatt ttccacatct ctattttctg cccgcagaat aatccaaact
     2341 gcaggctagc caccatgact tcgaaagttt atgatccaga acaaaggaaa cggatgataa
     2401 ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt tcttgattca tttattaatt
     2461 attatgattc agaaaaacat gcagaaaatg ctgttatttt tttacatggt aacgcggcct
     2521 cttcttattt atggcgacat gttgtgccac atattgagcc agtagcgcgg tgtattatac
     2581 cagaccttat tggtatgggc aaatcaggca aatctggtaa tggttcttat aggttacttg
     2641 atcattacaa atatcttact gcatggtttg aacttcttaa tttaccaaag aagatcattt
     2701 ttgtcggcca tgattggggt gcttgtttgg catttcatta tagctatgag catcaagata
     2761 agatcaaagc aatagttcac gctgaaagtg tagtagatgt gattgaatca tgggatgaat
     2821 ggcctgatat tgaagaagat attgcgttga tcaaatctga agaaggagaa aaaatggttt
     2881 tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa aatcatgaga aagttagaac
     2941 cagaagaatt tgcagcatat cttgaaccat tcaaagagaa aggtgaagtt cgtcgtccaa
     3001 cattatcatg gcctcgtgaa atcccgttag taaaaggtgg taaacctgac gttgtacaaa
     3061 ttgttaggaa ttataatgct tatctacgtg caagtgatga tttaccaaaa atgtttattg
     3121 aatcggaccc aggattcttt tccaatgcta ttgttgaagg tgccaagaag tttcctaata
     3181 ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga agatgcacct gatgaaatgg
     3241 gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa tgaacaataa ttctagagcg
     3301 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3361 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3421 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3481 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     3541 taaggatcca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3901 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4141 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4201 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4261 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4321 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4381 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4441 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4501 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4561 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4681 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4741 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4801 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4861 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4921 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4981 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5041 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5101 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5161 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5221 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5281 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5341 ctggcctttt gctcacatgg ctcgac
//
