LOCUS       sequence-141623-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged mNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-mNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
       61 gatgacaagg gatccggcgg cggcggcggc gccatgagtg tgggtcttcc tggtccccac
      121 agtttgccta gttctgagga agcatcgaat tctgggaacg cctcatcaat gcctgcagtt
      181 tttcatcccg agaactattc ttgcttacaa gggtctgcta ctgagatgct ctgcacagag
      241 gctgcctctc ctcgcccttc ctctgaagac ctgcctcttc aaggcagccc tgattcttct
      301 accagtccca aacaaaagct ctcaagtcct gaggctgaca agggccctga ggaggaggag
      361 aacaaggtcc ttgccaggaa gcagaagatg cggactgtgt tctctcaggc ccagctgtgt
      421 gcactcaagg acaggtttca gaagcagaag tacctcagcc tccagcagat gcaagaactc
      481 tcctccattc tgaacctgag ctataagcag gttaagacct ggtttcaaaa ccaaaggatg
      541 aagtgcaagc ggtggcagaa aaaccagtgg ttgaagacta gcaatggtct gattcagaag
      601 ggctcagcac cagtggagta tcccagcatc cattgcagct atccccaggg ctatctggtg
      661 aacgcatctg gaagcctttc catgtggggc agccagactt ggaccaaccc aacttggagc
      721 agccagacct ggaccaaccc aacttggaac aaccagacct ggaccaaccc aacttggagc
      781 agccaggcct ggaccgctca gtcctggaac ggccagcctt ggaatgctgc tccgctccat
      841 aacttcgggg aggactttct gcagccttac gtacagttgc agcaaaactt ctctgccagt
      901 gatttggagg tgaatttgga agccactagg gaaagccatg cgcattttag caccccacaa
      961 gccttggaat tattcctgaa ctactctgtg actccaccag gtgaaatatg a
//
