LOCUS       sequence-141626-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag tagged cNanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-Flag3-pG-cNanog-pgk-hph
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
       61 gatgacaagg gatccggcgg cggcggcggc gccatgccgt cctacggctc tgttaggtgc
      121 ggacgctact actggccctc tccgggcagc atggatagcg cgtctgccgc ggaagcccca
      181 gcagcagacc tctccttgac cacagagcag aaaacgccct gccacccaga tgcctctcca
      241 gcttcttcca gctctgggac actcattcag tataccccag actctgccac tagccctact
      301 gcagaccacc catctcaccg ccccactttt cagaaggtta aggataaagg tgagagtggg
      361 acaaggaagg ccaagagccg cacagctttc tcccaggagc agctgcagac cctgcaccag
      421 cggtttcaga gccagaagta cctcagcccc catcagatcc gggagctggc tgctgctctg
      481 gggctcacct acaagcaggt gaagacgtgg tttcagaacc aacgaatgaa gtttaaacgt
      541 tgccagaaag agagtcagtg ggtggataaa gggatttatc taccacagaa tgggtttcat
      601 caagctgcgt atctggatat gacccctacg tttcaccagg gcttccctgt tgttgccaac
      661 agaaaccttc aggctgtgac cagtgcacac caggcttaca gcagtggcca gacttatggg
      721 aatgggcagg gcctgtatcc gttcatggct gtggaggatg agggcttctt tggaaaaggt
      781 ggaacaagct gcaacgccca gcaggccatg ggtttattaa gtcaacagat gaacttctat
      841 catggctact ctaccaatgt ggattatgac agcttgcagg cagaagatac ctacagcttc
      901 cagagcacct ctgatagtat cacacagttc tcgagctctc ctgtacggca tcagtaccag
      961 gccccttggc ataccctggg gacccagaat ggttatgaga cttag
//
