LOCUS       sequence-141648-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-BFP-24xPP7
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(5105..5122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5111..5335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5381..5809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5468..5487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5678..5699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5818..5838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5823..6152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6003..6138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6065..6084
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6200..6247
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6266..6640
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6770..6891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6807..6826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6861..6880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6940..6956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6940..6956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6953..6975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6964..6980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6988..6994,6995..7012,7013..7018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7033..7054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7171..7188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7342..7927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7422..7441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8098..8889,8890..8958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8721..8740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8959..9063)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcatctgag ctgattaaag
     2821 aaaatatgca tatgaagctg tatatggagg ggacagtgga caatcaccat ttcaaatgta
     2881 catctgaagg agaggggaaa ccctatgaag gaacacagac aatgaggatc aaagtggtgg
     2941 aggggggccc cctgcctttc gcttttgaca ttctggctac cagctttctg tatggaagca
     3001 agactttcat caaccatacc cagggaattc ctgatttctt taaacagtcc ttccctgagg
     3061 gatttacatg ggaacgggtg acaacttacg aggacggcgg ggtgctgacc gccactcagg
     3121 atacaagcct gcaggacggc tgcctgatct ataatgtgaa gatcagaggc gtgaacttca
     3181 cttccaatgg ccccgtgatg cagaagaaga ccctgggctg ggaggcattc actgagactc
     3241 tgtacccagc tgacggggga ctggaagggc gcaacgatat ggcactgaaa ctggtggggg
     3301 gaagccatct gattgcaaac attaaaacta cataccgctc taaaaaaccc gcaaaaaacc
     3361 tgaaaatgcc aggcgtgtat tacgtggatt accgcctgga gcgcatcaaa gaggctaata
     3421 acgagaccta cgtggaacag cacgaggtgg ctgtggctcg ctactgcgat ctgccttcta
     3481 agctgggaca caaactgaac gatatctaac gacacgattc gaagatcgca tcgctagccc
     3541 ggttgtacag gtaccggatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     3601 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     3661 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     3721 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     3781 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     3841 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     3901 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
     3961 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
     4021 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
     4081 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     4141 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga
     4201 tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc
     4261 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg
     4321 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
     4381 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
     4441 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     4501 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     4561 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     4621 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
     4681 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
     4741 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
     4801 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
     4861 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
     4921 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
     4981 gctggctgca gtattcccgg gttcattaga tctcgcgaag ggcgaattca cgaggcgcgc
     5041 ccggatcgcg atcgcaatct taattaactc gagtctagag ggcccgttta aacccgctga
     5101 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5161 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5221 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5281 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5341 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5581 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5821 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5881 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5941 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6061 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6121 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6181 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     6241 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     6301 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     6361 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     6421 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     6481 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     6541 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     6601 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     6661 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6721 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7021 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7741 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7861 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8161 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9061 gcgcacattt ccccgaaaag tgccacctga cgtc
//
