LOCUS       sequence-141651-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1391..1400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1439..1495
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1511..1567
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1583..1639
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1655..1711
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1727..1783
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1799..1855
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1871..1927
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1943..1999
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2015..2071
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2087..2143
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2159..2215
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2231..2287
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2303..2359
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2375..2431
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2447..2503
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2519..2575
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2591..2647
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2663..2719
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2735..2791
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2807..2863
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2879..2935
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2951..3007
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3095..3151
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(7351..7368)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7357..7581
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7627..8055
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7714..7733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7924..7945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8064..8084)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8069..8398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8249..8384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8311..8330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8446..8493
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8512..8886
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9016..9137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9053..9072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9107..9126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9186..9202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9186..9202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9199..9221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9210..9226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9234..9240,9241..9258,9259..9264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9279..9300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9417..9434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9588..10173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9668..9687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10344..11135,11136..11204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10967..10986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11205..11309)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgttagga gggagagcag agcgcgcgcg taaatcctag
     1021 agaggcgggc taagctggac tggggggagg gtccgtcttc cggaaagtct ggattcccgg
     1081 acgagccgag ttgctgctca ccgaactccc gttcgagaga tgatcgaaga aagtcggcta
     1141 ccatttgtac ccatcaaaga tctccagatg gaagccagcg ctgaatttgg gctgagatta
     1201 ggacttgcag gaggccggtc cagaagacgg cggaaggaat cttggcgggc gcacgcatgc
     1261 gtgatagacc ctccacacgt gtggccgggc cgcggcctcc ccgtgctcgg aggtcccgcc
     1321 cccggccgta gcatctttcc ggacgtgggg agccggttgt aaagaagaaa gttctaccct
     1381 ttctgtcaaa gccaccatgg tcggtccggg tggatctgga ggtggaggtt ctggaggaga
     1441 agaacttttg agcaagaatt atcatcttga gaacgaagtg gctcgtctta agaaaggttc
     1501 tggcagtgga gaagaactgc tttcaaagaa ttaccacctg gaaaatgagg tagctagact
     1561 gaaaaagggg agcggaagtg gggaggagtt gctgagcaaa aattatcatt tggagaacga
     1621 agtagcacga ctaaagaaag ggtccggatc gggtgaggag ttactctcga aaaattatca
     1681 tctcgaaaac gaagtggctc ggctaaaaaa gggcagtggt tctggagaag agctattatc
     1741 taaaaactac cacctcgaaa atgaggtggc acgcttaaaa aagggaagtg gcagtggtga
     1801 agagctacta tccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagggttc
     1861 cggctcagga gaggaactgc tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt
     1921 aaaaaaggga tcgggcagtg gtgaggaact actttcaaag aattaccacc tcgaaaacga
     1981 agtagctcga ttaaagaaag gttcagggtc gggtgaagaa ttactgagta aaaattatca
     2041 tctggaaaat gaggtagcga gactaaaaaa ggggagtggt tctggcgaag agttgctatc
     2101 gaaaaattat catcttgaga acgaagttgc taggctcaaa aagggctcag gctcaggcga
     2161 ggagttgctc tcgaaaaact accacttgga aaatgaggtc gcgaggttga aaaaggggag
     2221 cgggtcgggc gaggagttat tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt
     2281 aaagaaaggc tcgggctcgg gcgaagaact cttatcgaag aactaccacc tcgaaaatga
     2341 ggtcgccagg ttgaaaaagg gcagtggcag cggggaggaa ctcttgagca agaactacca
     2401 cttggagaat gaggtcgcga gattgaagaa agggtcgggg agcggcgagg aattgctcag
     2461 caagaattat catttggaga acgaagtcgc caggctcaag aaaggctcgg ggtcggggga
     2521 ggaattgttg agtaaaaact accacttgga aaatgaagtc gccaggctca aaaaagggag
     2581 tgggagcggc gaagagttat tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct
     2641 caagaaaggg agcggcagcg gggaggagct cttatcgaag aactaccact tagagaatga
     2701 agtcgcccgc ttgaagaaag gctcggggag cggggaagag ctcttgagca agaactacca
     2761 cttggaaaat gaggtggcgc gcttgaagaa agggagcggg agcggggaag agttactatc
     2821 taagaattat catctcgaga acgaggtggc tcgactaaag aagggctccg gcagtgggga
     2881 ggaactcctg tcgaagaact atcatcttga aaatgaggtt gcaagactta aaaaggggtc
     2941 cggatcaggt gaggaactac tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt
     3001 gaagaaagga tcaggatcag gcgaagaact gctctcaaaa gattatcatt tggaaaatga
     3061 ggttgcacgt ttaaaaaagg gaagtggcag tggtgaggaa cttctgtcga aaaattatca
     3121 tctcgagaat gaagtagccc gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg
     3181 gtcagcagtg gaggacagca cgctgcaagt agtggtacgg gtgcggcccc ccacccctcg
     3241 ggagctggac agtcagcggc ggccagtggt tcaggtggtg gacgagcggg tgctggtgtt
     3301 taaccctgag gagcccgatg gagggttccc tggcctgaaa tggggtggca cccatgatgg
     3361 ccccaagaag aagggcaaag acctgacgtt tgtctttgac cgggtctttg gcgaggcggc
     3421 cacccaacag gacgtgttcc agcacaccac gcacagcgtc ctggacagct tcctccaggg
     3481 ctacaactgc tcagtgtttg cctacggggc caccggggct gggaagacac acaccatgct
     3541 gggaagggag ggggaccccg gcatcatgta cctgaccacc gtggaactgt acaggcgcct
     3601 ggaggcccgc cagcaggaga agcacttcga ggtgctcatc agctaccagg aggtgtataa
     3661 tgaacagatc catgacctcc tggagcccaa ggggcccctt gccatccgcg aggaccccga
     3721 caagggggtg gtggtgcaag gactttcttt ccaccagcca gcctcagccg agcagctgct
     3781 ggagatactg accaggggga accgtaaccg cacgcagcac cccactgatg ccaacgcgac
     3841 ttcctcccgc tcccatgcca tcttccagat ctttgtgaag cagcaggacc gggttccagg
     3901 actgacccag gctgtccagg tggccaagat gagcctgatt gacctggctg gctcagagcg
     3961 ggcatccagc acccatgcga agggggagcg gctgcgggag ggggccaaca tcaaccgctc
     4021 tctgctggcg ctcatcaacg tcctcaatgc cttggccgat gcaaagggcc gcaagaccgc
     4081 tgtgccctac gcggacagcg cactgacccg cctgctcaaa gactccctcg ggggcaactg
     4141 ccgcacagtg atgatcgctg ccatcagccc ctccagcctg acctacgagg acacgtataa
     4201 taccctcaaa tatgccgacc gggccaagga gatcaggctc tcgctgaaga gcaatgtgac
     4261 cagcctggac tgtcacatca gccagtatgc taccatctgc caacagctcc aggctgaggt
     4321 agccgctctg aggaagaagc tccaagtgta tgagggggga ggccagcccc caccacagga
     4381 cctcccagga tctcccaagt cgggaccacc accagaacac cttcccagct cccccttgcc
     4441 accccaccct cccagccagc cctgcacccc agagctccct gcagggccta gagcccttca
     4501 agaggagagt ctggggatgg aggcccaggt ggagagggcc atggaaggga actcttcaga
     4561 ccaggagcag tccccagagg atgaggatga aggcccagct gaggaggttc caacccagat
     4621 gccagagcag aaccccacac atgcactgcc agagtcccct cgcctgaccc tgcagcccaa
     4681 gccagtcgtg ggccacttct cagcacggga actggatggg gaccgttcta agcggttggc
     4741 cctaaaggtg ctgtgcgttg cccagcggca gtactccctg ctccaagcag ccaacctcct
     4801 gacgcccgac atgatcacag agtttgagac cctacagcag ctggtgcaag aggaaaaaat
     4861 tgagcctggg gcagaggcct tgaggacttc aggcctggcc aggggggcac ctctggctca
     4921 ggagctgtgt tcagagtcaa agcctccagg atacactggc cctgtgaccc ggactatggc
     4981 gaggcgactg agtggccccc tgcacaccct gggaatcccg cctggaccca actgcacccc
     5041 agcccagggg tcccgatggc ccatggagaa gaagaggagg agaccaagcg ccttggaggc
     5101 agacagtccc atggccccaa agcggggcac caagcgccag cgccagtcct tcctgccctg
     5161 cctaaggaga gggtctctgc ctgacaccca accttcacag gggcccagca cccccaaagg
     5221 agaaagggcc tcctccccct gccattcccc tcgcgtttgc ccagccacag tcatcaaaag
     5281 ccgggtgccc ctgggccctt ccgccatgca gaactgctcc accccgctgg ctctgcccac
     5341 tcgagacctc aatgccacct ttgatctctc tgaggagcct ccctcaaagc ccagtttcca
     5401 tgaatgcatt ggctgggaca aaatacccca ggagctgagc aggctggacc agcccttcat
     5461 ccccagggca cctgtgcccc tgttcaccat gaagggcccc aagccaacat cttccctccc
     5521 tgggacctct gcctgcaaga agaagcgcgt tgcgagttcc tcagtctccc atggccgcag
     5581 ccgcatcgcc cgcctcccca gcagcacttt gaagaggcca gctgggcccc ttgtactccc
     5641 agagctgccc ttgagtcccc tgtgccctag caaccggagg aatggaaagg acctcatcag
     5701 ggtggggaga gcactctcag cagggaacgg cgtcaccaag gtgtccgata tctaacgaca
     5761 cgattcgaag atcgcatcgc tagcccggtt gtacaggtac cggatcctaa ggtacctaat
     5821 tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc
     5881 agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa
     5941 ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag
     6001 cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta
     6061 attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca
     6121 gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct
     6181 aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc
     6241 agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc
     6301 taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac
     6361 cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac
     6421 ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa
     6481 ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta
     6541 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     6601 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     6661 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     6721 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     6781 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     6841 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     6901 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     6961 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     7021 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     7081 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     7141 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     7201 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatctc
     7261 gcgaagggcg aattcacgag gcgcgcccgg atcgcgatcg caatcttaat taactcgagt
     7321 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7381 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7441 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7501 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7561 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7621 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7681 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7741 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7801 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7861 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7921 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7981 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8041 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8101 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8161 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8221 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8401 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     8461 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     8521 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     8581 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     8641 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     8701 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     8761 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     8821 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     8881 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8941 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9001 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9121 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
    10141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
