LOCUS       sequence-141656-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: iLOV-Mito-7
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..90
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     polyA_signal    581..702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(618..637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     672..691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcagaatccg ctagcatgtc cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg
       61 gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggggatcc accggtcgcc
      121 accatgatcg agaagaactt cgtgatcacc gaccccagac tgcccgacaa ccccatcatc
      181 ttcgccagcg acggcttcct ggagctgacc gagtacagca gagaggagat cctgggcaga
      241 aacgccagat tcctgcaggg ccccgagacc gaccaggcca ccgtgcagaa gatcagagac
      301 gccatcagag accagagaga gaccaccgtg cagctgatca actacaccaa gagcggcaag
      361 aagttctgga acctgctgca cctgcagccc gtgagagacc agaagggcga gctgcagtac
      421 ttcatcggcg tgcagctgga cggcagcgac cacgtgtaag cggccgcgac tctagatcat
      481 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      541 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      661 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
      721 ttaatatttt gttaaaattc gcgttaaatt
//
