LOCUS       sequence-141657-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 450, Emission: 495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: iLOV-Mito-7
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..57
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     8..28
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             87..161
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
       61 ttagtgaacc gtcagaatcc gctagcatgt ccgtcctgac gccgctgctg ctgcggggct
      121 tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc
      181 caccggtcgc caccatgatc gagaagaact tcgtgatcac cgaccccaga ctgcccgaca
      241 accccatcat cttcgccagc gacggcttcc tggagctgac cgagtacagc agagaggaga
      301 tcctgggcag aaacgccaga ttcctgcagg gccccgagac cgaccaggcc accgtgcaga
      361 agatcagaga cgccatcaga gaccagagag agaccaccgt gcagctgatc aactacacca
      421 agagcggcaa gaagttctgg aacctgctgc acctgcagcc cgtgagagac cagaagggcg
      481 agctgcagta cttcatcggc gtgcagctgg acggcagcga ccacgtgtaa gcggccgcga
      541 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      601 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgtgt
//
