LOCUS       sequence-141666-        2590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2590)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(60..62,63..65,66..776)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(105..126)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(366..385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     713..734
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcacca
       61 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      121 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      181 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
      301 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      361 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      481 agctggagta caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg
      541 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      661 acctgagcta ccagtccaaa ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      721 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
      781 gactcagatc tcgagctcaa gcttcgatgg cgactgcgac ccccgtgccg ccgcggatgg
      841 gcagccgcgc tggcggcccc accacgccgc tgagccccac gcgcctgtcg cggctccagg
      901 agaaggagga gctgcgcgag ctcaatgacc ggctggcggt gtacatcgac aaggtgcgca
      961 gcctggagac ggagaacagc gcgctgcagc tgcaggtgac ggagcgcgag gaggtgcgcg
     1021 gccgtgagct caccggcctc aaggcgctct acgagaccga gctggccgac gcgcgacgcg
     1081 cgctcgacga cacggcccgc gagcgcgcca agctgcagat cgagctgggc aagtgcaagg
     1141 cggaacacga ccagctgctc ctcaactatg ctaagaagga atctgatctt aatggcgccc
     1201 agatcaagct tcgagaatat gaagcagcac tcaattcgaa agatgcagct cttgctactg
     1261 cacttggtga caaaaaaagt ttagagggag atttggagga tctgaaggat cagattgccc
     1321 agttggaagc ctccttagct gcagccaaaa aacagttagc agatgaaact ttacttaaag
     1381 tagatttgga gaatcgttgt cagagcctta ctgaggactt ggagtttcgc aaaagcatgt
     1441 atgaagagga gattaacgag accagaagga agcatgaaac gcgcttggta gaggtggatt
     1501 ctgggcgtca aattgagtat gagtacaagc tggcgcaagc ccttcatgag atgagagagc
     1561 aacatgatgc ccaagtgagg ctgtataagg aggagctgga gcagacttac catgccaaac
     1621 ttgagaatgc cagactgtca tcagagatga atacttctac tgtcaacagt gccagggaag
     1681 aactgatgga aagccgcatg agaattgaga gcctttcatc ccagctttct aatctacaga
     1741 aagagtctag agcatgtttg gaaaggattc aagaattaga ggacttgctt gctaaagaaa
     1801 aagacaactc tcgtcgcatg ctgacagaca aagagagaga gatggcggaa ataagggatc
     1861 aaatgcagca acagctgaat gactatgaac agcttcttga tgtaaagtta gccctggaca
     1921 tggaaatcag tgcttacagg aaactcttag aaggcgaaga agagaggttg aagctgtctc
     1981 caagcccttc ttcccgtgtg acagtatccc gagcatcctc aagtcgtagt gtacgtacaa
     2041 ctagaggaaa gcggaagagg gttgatgtgg aagaatcaga ggcgagtagt agttagcatc
     2101 tctcattccg cctcagccac tggaaatgtt tgcatcgaag aaattgatgt tgatgggaaa
     2161 tttatccgct tgaagaacac ttctgaacag gatcaaccaa tgggaggctg ggagatgatc
     2221 agaaaaattg gagacacatc agtcagttat aaatatacct caagatatgt gctgaaggca
     2281 ggccagactg ttacaatttg ggctgtgcaa acgctggtgt cacagccagc cccccaactg
     2341 acctcatctg gaagaaccag aactcgtggg gcactggcga agatgtgaag gttatattga
     2401 aaaattctca gggagaggag gttgctcaaa gaagtacagt ctttaaaaca accatacctg
     2461 aagaagagga ggaggaggaa gaagcagctg gagtggttgt tgaggaagaa cttttccacc
     2521 agcagggaac cccaagagca tccaatagaa gctgtgcaat tatgtaggga tccaccggat
     2581 ctagataact
//
