LOCUS       sequence-141680-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      240..249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             246..929
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag
       61 atctggtacc ccttgcagct tccagttgtc aggaagagaa ggctcccatg gatcctgtgg
      121 tggtgctggg gctctgtctc tcctgtttgc ttctcctttc actctggaaa cagagctatg
      181 ggggagggaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg
      241 ccaccatggt gagtgcgatt aagccagaca tgaggatcaa actccgtatg gaaggcaacg
      301 taaacgggca ccactttgtg atcgacggag atggtacagg caagccttat gagggaaaac
      361 agaccatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      421 ccactgcatt ccattacggc aacagggtat tcgttaaata tccagacaac atacaagact
      481 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      541 ggggcatttg ctatgccaga aacgacataa caatggaagg ggacactttc tataataaag
      601 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      661 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      721 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
      781 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac gccattgaga
      841 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
      901 ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat aatcagccat
      961 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1021 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgca
//
