LOCUS       sequence-141682-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-encoding vector for marker replacement mutagenesis in 
            Bradyrhizobium diazoefficiens and other Alphaproteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pREDSIX
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(336..353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(587..606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
       61 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      121 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      181 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      241 aggaattgaa gcttagatct tgactgccag ctgcattaat gaatcggcca acgcgcgggg
      301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      961 atctgcgctc tgctgaagcc agttaccttc ggaaa
//
