LOCUS       sequence-141683-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-encoding vector for marker replacement mutagenesis in 
            Bradyrhizobium diazoefficiens and other Alphaproteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pREDSIX
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        396..500
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(719..738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac cataattata
      121 tctccttctt atttctagtc aagatctaag cttcgtgcaa tcctaccgcg ggaccggcca
      181 taagccgggc gtaaaggcgc ggaccgatta gaggcttgtg cgacgatgat caagcggcag
      241 gtttgccggc agggcggatc gcgccgaatg aggggtcagg acgcggcctc gccgacctgc
      301 tcgtagagcc gctgcgcctc cggggcgccg ccgcggcgct cgccgagatc gagcaagctt
      361 acctttgtct tcactaaggg cgaattggaa atgtgcgcgg aacccctatt tgtttatttt
      421 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      481 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      541 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      601 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      661 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      721 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      781 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      841 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      901 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      961 gggatcatgt aactc
//
