LOCUS       sequence-141694-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of hVEGF165 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Marshall WG Jr, Boone BA, Burgos JD, Gografe SI, Baldwin MK, 
            Danielson ML, Larson MJ, Caretto DR, Cruz Y, Ferraro B, Heller LC, 
            Ugen KE, Jaroszeski MJ, Heller R
  TITLE     Electroporation-mediated delivery of a naked DNA plasmid expressing 
            VEGF to the porcine heart enhances protein expression.
  JOURNAL   Gene Ther. 2010 Mar;17(3):419-23. doi: 10.1038/gt.2009.153. Epub 
            2009 Dec 3.
  PUBMED    19956270
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2009.153"; journalName: "Gene Ther"; date: "2010-03"; 
            volume: "17"; issue: "3"; pages: "419-23"
COMMENT     Sequence Label: pVax1-hVEGF165
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(697..714)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    703..814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctca tgaactttct gctgtcttgg gtgcattgga
      121 gccttgcctt gctgctctac ctccaccatg ccaagtggtc ccaggctgca cccatggcag
      181 aaggaggagg gcagaatcat cacgaagtgg tgaagttcat ggatgtctat cagcgcagct
      241 actgccatcc aatcgagacc ctggtggaca tcttccagga gtaccctgat gagatcgagt
      301 acatcttcaa gccatcctgt gtgcccctga tgcgatgcgg gggctgctgc aatgacgagg
      361 gcctggagtg tgtgcccact gaggagtcca acatcaccat gcagattatg cggatcaaac
      421 ctcaccaagg ccagcacata ggagagatga gcttcctaca gcacaacaaa tgtgaatgca
      481 gaccaaagaa agatagagca agacaagaaa atccctgtgg gccttgctca gagcggagaa
      541 agcatttgtt tgtacaagat ccgcagacgt gtaaatgttc ctgcaaaaac acagactcgc
      601 gttgcaaggc gaggcagctt gagttaaacg aacgtacttg cagatgtgac aagccgaggc
      661 ggtgagctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
      721 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      781 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      841 ttctggg
//
