LOCUS       sequence-141695-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of hVEGF165 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Marshall WG Jr, Boone BA, Burgos JD, Gografe SI, Baldwin MK, 
            Danielson ML, Larson MJ, Caretto DR, Cruz Y, Ferraro B, Heller LC, 
            Ugen KE, Jaroszeski MJ, Heller R
  TITLE     Electroporation-mediated delivery of a naked DNA plasmid expressing 
            VEGF to the porcine heart enhances protein expression.
  JOURNAL   Gene Ther. 2010 Mar;17(3):419-23. doi: 10.1038/gt.2009.153. Epub 
            2009 Dec 3.
  PUBMED    19956270
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2009.153"; journalName: "Gene Ther"; date: "2010-03"; 
            volume: "17"; issue: "3"; pages: "419-23"
COMMENT     Sequence Label: pVax1-hVEGF165
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(593..612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(657..756)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(686..706)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acatctgcaa gtacgttcgt ttaactcaag ctgcctcgcc ttgcaacgcg agtctgtgtt
       61 tttgcaggaa catttacacg tctgcggatc ttgtacaaac aaatgctttc tccgctctga
      121 gcaaggccca cagggatttt cttgtcttgc tctatctttc tttggtctgc attcacattt
      181 gttgtgctgt aggaagctca tctctcctat gtgctggcct tggtgaggtt tgatccgcat
      241 aatctgcatg gtgatgttgg actcctcagt gggcacacac tccaggccct cgtcattgca
      301 gcagcccccg catcgcatca ggggcacaca ggatggcttg aagatgtact cgatctcatc
      361 agggtactcc tggaagatgt ccaccagggt ctcgattgga tggcagtagc tgcgctgata
      421 gacatccatg aacttcacca cttcgtgatg attctgccct cctccttctg ccatgggtgc
      481 agcctgggac cacttggcat ggtggaggta gagcagcaag gcaaggctcc aatgcaccca
      541 agacagcaga aagttcatga gcttaagttt aaacgctagc cagcttgggt ctccctatag
      601 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      661 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      721 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      781 tcaatggg
//
