LOCUS       sequence-141699-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mGM-CSF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R
  TITLE     In vivo electroporation of plasmids encoding GM-CSF or interleukin-2
            into existing B16 melanomas combined with electrochemotherapy 
            induces long-term antitumour immunity.
  JOURNAL   Melanoma Res. 2000 Dec;10(6):577-83.
  PUBMED    11198480
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Melanoma 
            Res."; date: "2000-12"; volume: "10(6)"; pages: "577-83"
COMMENT     Sequence Label: pCR3.1-mGM-CSF
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(665..682)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    671..782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
      121 tcggcttctg aggaggatgt ggctgcagaa tttacttttc ctgggcattg tggtctacag
      181 cctctcagca cccacccgct cacccatcac tgtcacccgg ccttggaagc atgtagaggc
      241 catcaaagaa gccctgaacc tcctggatga catgcctgtc acgttgaatg aagaggtaga
      301 agtcgtctct aacgagttct ccttcaagaa gctaacatgt gtgcagaccc gcctgaagat
      361 attcgagcag ggtctacggg gcaatttcac caaactcaag ggcgccttga acatgacagc
      421 cagctactac cagacatact gccccccaac tccggaaacg gactgtgaaa cacaagttac
      481 cacctatgcg gatttcatag acagccttaa aacctttctg actgatatcc cctttgaatg
      541 caaaaaacca ggccaaaaat gaggaagccc aggccagctc tgaatccaga agccgaattc
      601 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
      661 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      721 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      781 tcgcattgtc tgagtaggtg tcattctatt ctggg
//
