LOCUS       sequence-141700-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mGM-CSF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R
  TITLE     In vivo electroporation of plasmids encoding GM-CSF or interleukin-2
            into existing B16 melanomas combined with electrochemotherapy 
            induces long-term antitumour immunity.
  JOURNAL   Melanoma Res. 2000 Dec;10(6):577-83.
  PUBMED    11198480
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Melanoma 
            Res."; date: "2000-12"; volume: "10(6)"; pages: "577-83"
COMMENT     Sequence Label: pCR3.1-mGM-CSF
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(583..602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(584..602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(647..746)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(676..696)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agcggccgcc actgtgctgg atatctgcag aattcggctt ctggattcag agctggcctg
       61 ggcttcctca tttttggcct ggttttttgc attcaaaggg gatatcagtc agaaaggttt
      121 taaggctgtc tatgaaatcc gcataggtgg taacttgtgt ttcacagtcc gtttccggag
      181 ttggggggca gtatgtctgg tagtagctgg ctgtcatgtt caaggcgccc ttgagtttgg
      241 tgaaattgcc ccgtagaccc tgctcgaata tcttcaggcg ggtctgcaca catgttagct
      301 tcttgaagga gaactcgtta gagacgactt ctacctcttc attcaacgtg acaggcatgt
      361 catccaggag gttcagggct tctttgatgg cctctacatg cttccaaggc cgggtgacag
      421 tgatgggtga gcgggtgggt gctgagaggc tgtagaccac aatgcccagg aaaagtaaat
      481 tctgcagcca catcctcctc agaagccgaa ttccaccaca ctggactagt ggatccgagc
      541 tcggtaccaa gcttaagttt aaacgctagc cagcttgggt ctccctatag tgagtcgtat
      601 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      661 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      721 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      781 ggagacttgg aa
//
