LOCUS       sequence-141701-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse IL2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R
  TITLE     In vivo electroporation of plasmids encoding GM-CSF or interleukin-2
            into existing B16 melanomas combined with electrochemotherapy 
            induces long-term antitumour immunity.
  JOURNAL   Melanoma Res. 2000 Dec;10(6):577-83.
  PUBMED    11198480
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Melanoma 
            Res."; date: "2000-12"; volume: "10(6)"; pages: "577-83"
COMMENT     Sequence Label: pCR3.1-mIL2
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(787..804)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc ggcttccttg ctaatcactc ctcacagtga cctcaagtcc tgcaggcatg
      181 tacagcatgc agctcgcatc ctgtgtcaca ttgacacttg tgctccttgt caacagcgca
      241 cccacttcaa gctccacttc aagctctaca gcggaagcac agcagcagca gcagcagcag
      301 cagcagcagc agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg
      361 atggagaatt acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc
      421 aagcaggcca cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg
      481 catgttctgg atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc
      541 agcaatatca gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa
      601 ttcgatgatg agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa
      661 agcatcatct caacaagccc tcaataacta tgtacctcct gcttacaaca caagccgaat
      721 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
      781 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tccc
//
