LOCUS       sequence-141702-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse IL2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R
  TITLE     In vivo electroporation of plasmids encoding GM-CSF or interleukin-2
            into existing B16 melanomas combined with electrochemotherapy 
            induces long-term antitumour immunity.
  JOURNAL   Melanoma Res. 2000 Dec;10(6):577-83.
  PUBMED    11198480
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Melanoma 
            Res."; date: "2000-12"; volume: "10(6)"; pages: "577-83"
COMMENT     Sequence Label: pCR3.1-mIL2
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(687..706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(751..802)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(780..800)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actgtgctgg atatctgcag aattcggctt gtgttgtaag caggaggtac atagttattg
       61 agggcttgtt gagatgatgc tttgacagaa ggctatccat ctcctcagaa agtccaccac
      121 agttgctgac tcatcatcga attggcactc aaatgtgttg tcagagccct ttagttttac
      181 aacagttact ctgatattgc tgatgaaatt ctcagcatct tccaattgaa agcttttgct
      241 ttgagtcaaa tccagaacat gccgcagagg tccaagttca tcttctaggc actgaagatc
      301 tttcaattct gtggcctgct tgggcaagta aaatttgaag gtgagcatcc tggggagttt
      361 caggttcctg taattctcca tcctgctcag gagctcctgt aggtccatca acagctgctc
      421 caggtgctgc tgctgctgct gctgctgctg ctgctgctgc tgtgcttccg ctgtagagct
      481 tgaagtggag cttgaagtgg gtgcgctgtt gacaaggagc acaagtgtca atgtgacaca
      541 ggatgcgagc tgcatgctgt acatgcctgc aggacttgag gtcactgtga ggagtgatta
      601 gcaaggaagc cgaattccac cacactggac tagtggatcc gagctcggta ccaagcttaa
      661 gtttaaacgc tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca
      721 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac
      781 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
//
