LOCUS       sequence-141706-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Histone H2B, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-H2B-6
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..468
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(793..812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtttagtg gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc
      121 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
      181 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
      241 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt
      301 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
      361 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
      421 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga tccaccggtc
      481 gccaccatgg tgagcaaagg cgaagagctg ttcaccggcg tggtgcccat cctggtggag
      541 ctggacggcg acgtgaacgg ccacaagttc agcgtgagcg gcgagggcga gggcgacgcc
      601 acctacggca agctgaccct gaagctgctg tgcaccaccg gcaagctgcc cgtgccctgg
      661 cccaccctgg tgaccaccct gggctacggc gtgcagtgct tcgcccggta ccccgaccac
      721 atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca ggagcggacc
      781 atcttcttca aggacgacgg caactacaag acccgggccg aggtgaagtt cgagggcga
//
