LOCUS       sequence-141708-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Secondary module (BpiI) site in pTU2b backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2_2b
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     655..674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
       61 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
      121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      721 gtgatgctcg tcaggg
//
