LOCUS       sequence-141709-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..138)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccaaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc tcgaggtcga
       61 gaattcttat tacttgtcat cgtcatcctt gtaatcaatg tcatgatctt tataatcacc
      121 gtcatggtct ttgtagtcgc ctgcggccgc tcaggtcatg tagcgggtga tggcacggag
      181 ggcgtacagc agagcggctt gcatgcgcgc ctccaggagc agcaagttca cgtggacctt
      241 cgctgagtgg cggaagtgcc tccagaagag gttgtacatt cttcgggtgg tcttctctgg
      301 gtagcaggcc actgtcttga tatagacctt gaggttccgc tccaggagct ggttcacctc
      361 cccataatca tagtcatcat atctgatgcc aaagacgcag tggatatagt tccagatggc
      421 cctgcggagc acggaggtgt ccacaccact gtgcatggcg atggtattgt aggtgaggct
      481 ataggctgcc tggaacttct catccagcag ctgcccaccc tcagggtaaa gccgctggat
      541 cagcgagtag ccatggtctt cccaggtata atcctgggcc cggaaggtag ggggtgcctg
      601 agcccctctc cgagtgaagt cctcatatcc gaaagtaggg tcttccacaa agcacagcat
      661 gtctgggtgt ggagagggct ccaggatgtc agctgagggg gtcaccagca ggctctctga
      721 cttctccagc tcaaagcggc tctccatctc ctcctgggac gtcccctcat cccgcagcag
      781 gctctcctgc agctgctgca tgcgctccat cagcgcctcc acgtcgcggg cagactcaaa
      841 gcccccagag ttgttcaacg ggtccctgct tggggggctg ctctgttcac ta
//
