LOCUS       sequence-141718-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing kanamycin resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-KmR
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(139..155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(148..170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(364..383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     483..505
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(543..561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        629..733
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
       61 tcttctgagc gggactctgg ggttcgccca agctatcact agtgaatcat cgatggtacc
      121 atatgttcga agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
      301 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
      361 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
      421 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
      481 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
      541 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      721 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      781 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctga
//
