LOCUS       sequence-141720-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing kanamycin resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-KmR
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(406..423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(406..422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(415..437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
       61 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      121 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
      181 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
      241 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
      301 acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagcta
      361 gatcttcgaa cgatactagt catatgaatt cggtaccagc ttggcactgg ccgtcgtttt
      421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      541 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
      601 tatttcacac cgcataactg gctgctat
//
