LOCUS       sequence-141722-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human HHEX .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_HHEX
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..110)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(126..149)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(180..203)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccaggtcca
       61 gggttctcct ccacgtctcc agcctgcttc agcaggctga agttagtagc tccgcttcct
      121 gaaaccttat cgtcgtcatc cttgtaatct cccttatcgt cgtcatcctt gtaatcgccc
      181 ttatcgtcgt catccttgta atctccagaa ccacctccac cggaacctcc tccgctccct
      241 ccagcattaa aatagctttt atcgccctca atgtccactt cctgatcaga atcctctgaa
      301 atctctgatt caaggtcttc ctgggaggca ggggagggcg aacattgaga gctatccaaa
      361 gaagcacctt tattctgttc actgggcaaa tcttgcctct gatcacagga actgtccaaa
      421 ctttccagtt cttctttttt attgctttga gggttctcct gaataaagga aatgaaaatg
      481 gagattggaa ggatcataaa atatggatag gtgagaggaa taaatcaaaa tgtctcttca
      541 tcctattcca tcttttaatt tttagtttac tgagtgtttt cagtttataa tcttattcag
      601 gagatacata cttgtgcttt ttgctcactt tggaagaaaa gtttctgtca tatcaaagat
      661 tatatgctta aaagatgaga accagtcatg aacataaggg cagatacatt ataccaattg
      721 aacacaaatt ctggttactt aacaccagcc cagacccatg gtttcaatat tctcaaaagt
      781 ttaaggggga aagaaaatca gatcactaca gatacacctt aacttcattc tttaaatatt
      841 taaaccttgc caattaacag tctttcagac attttaaaaa taagaatgct aactggtgaa
      901 tta
//
