LOCUS       sequence-141723-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing gentamicin resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-GmR
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(195..200,201..217,218..223))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gtacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
       61 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
      121 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
      181 gctacttgca ttacagttta cgaaccgaac aggcttatgt caattcgaga attaattcga
      241 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
      301 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
      361 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
      421 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
      481 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
      541 tagcgagggc tttactaagc tgatccggtg gatgaccttt tgaatgacct ttaatagatt
      601 atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac
      661 aaaacggttt acaagcataa agctagatct
//
