LOCUS       sequence-141725-         426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing gentamicin resistance gene cassette flanked by 
            multiple restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Ledermann R, Strebel S, Kampik C, Fischer HM
  TITLE     Versatile vectors for efficient mutagenesis of Bradyrhizobium 
            diazoefficiens and other Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
  PUBMED    26921431
REFERENCE   2  (bases 1 to 426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15."
COMMENT     Sequence Label: pRGD-GmR
FEATURES             Location/Qualifiers
     source          1..426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(360..377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(360..376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ataattcgtt caagccgaga tcggcttccc ggccaattcg acgcgcggat cagtgagggt
       61 ttgcaactgc gggtcaagga tctggatttc gatcacggca cgatcatcgt gcgggagggc
      121 aagggctcca aggatcgggc cttgatgtta cccgagagct tggcacccag cctgcgcgag
      181 caggggaatt gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
      241 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta
      301 caagcataaa gctagatcac tagtgaatca tcgatggtac catatgttcg aagcttggca
      361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      421 cttgca
//
