LOCUS       sequence-141726-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      780..789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             789..812
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggttcagtt ctataattag cagcaccgga taggacgcga gccaaacctc tcttaaccct
       61 agccagtccc tccaggaact gaaaagatga agaattcatt taggagattt cacactcggc
      121 tgtttggcct agcccagtga agtccctttt catcacgcaa ccctaatgac tcttagggct
      181 gtcactgccg tctggtgttg ccagggatct gactaaaccg tcttgcaaaa gtttgcagca
      241 ttcacccgag gcggactaca actcccagaa ttccgggcgc tccccggtgc tgattggcgg
      301 agcccgtgac tccagggggc ggagtttccc ccgcctcccc ggggaaacag acctctgatt
      361 ggctggaccg ccgggctttt gtgtccccgc cctcccccgg ggaccgcagc gggctgaact
      421 gctggtgtca gagcccggcg agcgctggca gttccgcggc ggggatgctg aggagcgctg
      481 ggtccgggag cagccctggc ccctgcggac ttccgaggcc gtgaaaaccc ctgcgctgcg
      541 gcccttccca ggcccccgag gccgttcgcc gttcccgaag cccgactggg ggaagagtcc
      601 agcaccaaag cggccgttct cggattccgg agcgttctgg agccccgaga gacgccccgg
      661 ggttctagaa gctccccggc ggcgcccagt cccggcttca ttcgggcgtc cctccgaaac
      721 ccactcgggt gcacgggtcg tcggcgagcc gcgaccgggt cctggcgcgc accaccggtg
      781 ccaccatgga ctacaaggat gacgatgaca agggtggtgg tggtggtgcg tccacgatga
      841 tcgtggcgga ctccgagtgc cgcgcagagc tcaaggacta cctgcggttc gcccc
//
