LOCUS       sequence-141731-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pJFRC7-RFPnls-PQRv2-mCD8:GFP
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..820)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(147..167)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     762..790
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat
       61 gtcacaccac agaagtaagg ttccttcaca aagatcctct agattatttg tatagttcat
      121 ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct
      181 tttcgttggg atctttcgaa agggcagatt gtgtggacag gtaatggttg tctggtaaaa
      241 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc
      301 catcttcaat gttgtgtcta attttgaagt taactttgat tccattcttt tgtttgtctg
      361 ccatgatgta tacattgtgt gagttatagt tgtattccaa tttgtgtcca agaatgtttc
      421 catcttcttt aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa
      481 acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct
      541 ggacataacc ttcgggcatg gcactcttga aaaagtcatg ctgtttcata tgatctgggt
      601 atcttgaaaa gcattgaaca ccataagtta aagtagtgac aagtgttggc catggaacag
      661 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt
      721 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
      781 ttgggacaac tccagtgaaa agttcttctc ctttactcat ggatccgcgg ctgtggtagc
      841 agatgagagt gatgatcaag gacagcagaa gggccacgca gattccggcc aagggtgccc
      901 agatgtaaat atcacaggcg aagtccaatc cggtcccctt cactgagcca cggggccgac
      961 aatcttctgg tctctggggc tgagatgtcc cggtagggtg cacaggtgag ggagttcgca
//
