LOCUS       sequence-141739-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express HA tagged optimized C9ORF72 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     pcDNA3 HA C9ORF72 op
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDNA3 HA C9ORF72 op
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gagtacccat acgatgttcc agattacgct aagcttatgt
      121 caaccctgtg ccctccaccc tcccctgctg tcgcaaaaac tgaaatcgcc ctgagcggaa
      181 aatcacccct gctggctgct accttcgctt actgggataa catcctggga cctcgggtgc
      241 gccacatttg ggcaccaaag accgaacagg tgctgctgag cgacggcgag atcacatttc
      301 tggccaacca tactctgaat ggagaaatcc tgaggaatgc agagtccggg gccattgacg
      361 tgaaattctt tgtgctgtcc gagaagggag tcatcatcgt gagcctgatc ttcgatggca
      421 actggaatgg agacagatca acctacgggc tgagcatcat tctgccacag acagagctga
      481 gcttttatct gcccctgcac cgcgtctgcg tggatcgact gacacatatc attcgaaaag
      541 gcaggatctg gatgcacaag gagcgacagg aaaacgtgca gaagatcatt ctggagggga
      601 cagaacgcat ggaggaccag ggccagtcca tcattcccat gctgactggc gaagtcattc
      661 ctgtgatgga gctgctgagc agcatgaaga gtcattcagt gcctgaggaa atcgatattg
      721 ctgacaccgt gctgaacgac gatgacatcg gagattcttg ccacgaaggg ttcctgctga
      781 atgccatttc tagtcatctg cagacatgcg gctgtagtgt ggtcgtgggc agcagcgccg
      841 agaaggtcaa caagatcgtg aggaccctgt gcctgtttct gacaccagcc gaaagaaaat
      901 gcagcaggct gtgcgaggct gaatcctctt tca
//
